Single molecule nanotechniques have emerged as powerful tools to investigate the topographies, forces and motions at the nanoscale - on samples as small as proteins and as large as cells - in both air and liquid associated with biological system. The most common, remarkable microscopy and force spectroscopy techniques applied in nanomanipulation and detection are atomic force microscopy (AFM), optical/laser tweezers, magnetic tweezers and single molecule high resolution fluorescence spectroscopy, etc. in our molecule imaging facility department of NCPSS. We focus on supporting issues that the most protein sciences in biology could be handled and cured precisely with these nanotechniques. It would be contributed largely to the development of industrial single molecule nanobiotechniques and further clinically genetic analysis & most protein science studies, which should largely support the protein science researchers from all over the country and world.
AFM provides the highest sensitivity, resolution and most accurate images and force measurements possible on all three axes ensure distortion. AFM is the only system capable of imaging complex samples with very rough topographies at the nanoscale. Sample loading and unloading and tip exchange are faster and more intuitive, and the post-data collection processing capabilities are clearly superior.
High resolution dual trap optical tweezers is able to manipulate single macromolecules and measure the extension and force. Applications involve the study of protein folding and protein-DNA interaction in single molecule level.
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